flow cytometry online course

Are you buying a flow cytometry online course? If not, you’re at least thinking about buying one. Why? The best flow cytometry online courses offer much more than just online learning (in comparison to the many other online courses). They are proved to increase practical skills and knowledge of learning. No wonder why these top-notch courses are the best sellers.

Cytofluorometry is a technique that consists in using a combination of light and aflorescence. Flourescence if focused to detect cells flowcytometry. This method is the most common used in cancer research and studies involving the hematopoietic system. The advantage of flow cytometry online course is easy to apply and useful.

Build up your confidence in flow cytometry analysis with the Abcam training series, with a certificate at the end. Whether you are new to flow cytometry or looking for optimization advice, our free training will help you achieve the most with this powerful technique.

Enroll for free flow cytometry training.

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Flow cytometry training structure

Our training introduces you to flow cytometry basics and essential protocols before moving on to optimization and troubleshooting.

The flow cytometry training is divided into 4 parts:

  • Part 1 guides you through the flow cytometry basics. You’ll learn how the flow cytometer works and how to select the right fluorophore for your experiment.
  • Part 2 covers sample preparation, appropriate controls, and staining protocols for flow cytometry analysis.
  • Part 3 teaches you how to design and run a flow cytometry experiment, set up compensation controls for multicolor flow cytometry, and visualize your data.
  • Part 4 shares with you troubleshooting tips for different issues you’re likely to encounter in flow cytometry.

Upon training completion, you can test your knowledge with our final quiz and get a certificate.

Enroll now and instantly access all training materials.

Get the best possible data from your cells.

Figure 1. Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-His tagged TMEM119 expression vector labeling TMEM119 with ab210405 at 1/2000 dilution (0.1μg/mL) (right) compared with isotype control rabbit monoclonal IgG ab172730 (Left). Only Myc-tag (+) population showed TMEM119 positive staining.

Flow cytometry training value

Our free online training guides you through all the essentials for flow cytometry analysis.

At the end of this training, you’ll be fully prepared to design and run flow cytometry experiments. You’ll also learn to:

  • Understand the flow cytometry basics
  • Prepare good samples for flow cytometry analysis
  • Select the appropriate controls
  • Set up compensation for multicolor flow cytometry analysis
  • Carry out and optimize protocols for direct, indirect and intracellular staining
  • Visualize and analyze your data
  • Troubleshoot the most common problems in flow cytometry analysis

Sign up now for our free on-demand flow cytometry training.

Already flowing in flow cytometry? Check out our training programs on antibody basicsfluorescent imagingwestern blotIHC, and ChIP.

Introduction to Flow Cytometry (online) – All FacultiesDuration3 hours (2 hours self study & 1 hour webinar)TeamResearcher Academy

This online course is run by the Researcher Academy in conjunction with the University of Nottingham Flow Cytometry Facility.
Course Description:

Provided by the University of Nottingham Flow Cytometry Facility this course is designed to provide a foundation knowledge in flow cytometric analysis.  

This is an online version of the classroom-based course ‘Introduction to Flow Cytometry (MHSIFC)’. The online version comprising 2 parts, firstly a self-directed study of the tutor’s lecture capture (duration 2 hrs and 2 minutes) followed by a webchat (1 hour).Part 1: Lecturerecording (asynchronous delivery):

Organised into two recording sessions: the first half will focus on theoretical background, common applications and cell sorting; while the second sessions will cover experimental design and compensation. 
Session 1 

  • What is flow cytometry and how does it work? 
  • Applications of flow cytometry in Biomedical Research. 
  • What is cell sorting and how does it work? 
  • How can I access flow cytometry technology and support at the University of Nottingham? 

Session 2 

  • Reagents for flow cytometry: Antibodies and Fluorochromes. 
  • Basic design of flow cytometry experiment. 
  • Colours that overlap and compensate. 
  • Information on further reading and links software and useful websites. 

Part 2: Live Webchat (synchronous delivery):

This is an opportunity to pose any questions arising from the lecture capture materials directly to the tutor and for interactive group discussion of concepts, challenges and guidance with the tutor and other users. Course learning outcomes:

  • Understand the basic concept of how cells are analysed using flow cytometry. 
  • Identify commonly used application(s) of flow cytometry in the student’s research field. Understanding the concept of cells sorting.
  • Ability to interpret basic flow cytometry data in scientific literature. 
  • Know how to access the Faculty Flow Cytometry Facility for further information, bookings, training and support.
  • Be aware of the range of fluorescent labels available and commonly used reagents.
  • Be able to design basic flow cytometry experiments, with awareness of controls appropriate to common applications
  • Understand compensation
  • Have details of helpful reference material. 

A two part, blended delivery online course comprising, 

  1. Self-directed study: watch lecture recording (duration 2 hours) 
  2. Live Webchat: tutor led discussion and Q&A (duration 1 hour)  

Note, the scheduled timings for the course instance are for the live synchronous delivery (webchat). The self-directed study must be completed in advance of this scheduled time. The overall course duration is 3 hours.
Platforms: Moodle and Microsoft Teams. Course booking confirmation email contains the Moodle page link, this link deactivates 2 hours before live webchat start time.

Course Accessibility

The following table shows a summary of what is needed to participate in the course. 

If you feel you will experience any difficulties participating, please let us know via the ‘special requirements’ tab, providing as much information as possible. The special requirements tab can be completed when you book your place. Alternatively, you can contact us directly at [email protected].

Access and/or complete self-study/ pre-course tasks prior to live webchat and Q&A
Access Moodle
Access MS Teams
Attend the live webchat and Q&A at the specified date and time
Use MS Teams chat box function during the live webchat
Engage with online materials
Watch and listen to pre-recorded videos
Watch and listen to the course tutor(s) and/or other attendees
Follow presentation slides during the course

Booking Conditions

Our webinars/ online courses are very popular, especially in the current period and we are experiencing large volumes of people on our waiting lists.  

Please do let us know if you would like to cancel your place on this course so we can offer your place to another delegate. 

Please note, attendances for courses will be recorded. There are no training points associated with courses run by the Researcher Academy.

Flow Cytometry Web-Based Training from BD Biosciences

BD Biosciences logo

1. Overview – 5:01

  • Define the term flow cytometry.
  • Describe the types of samples suitable for a flow cytometer.
  • Explain the properties that a flow cytometer can measure.
  • Identify the three subsystems of a flow cytometer.

2. Fluidics – 3:30

  • Describe the functions of the fluidics system.
  • Explain hydrodynamic focusing.
  • Describe how sample pressure affects flow rate and data resolution.

3. Optics – 7:48

  • Describe the functions of the optics system.
  • Discuss the role of lasers in BD cytometers.
  • Explain how filters route light to appropriate detectors.

4. Electronics – 6:59

  • Describe how the electronics system converts light signals into numerical data.
  • Explain how laser time delays ensure that signals from multiple lasers are attributed to the correct event.
  • Explain how and why thresholds are used.

5. Optical measurements – 9:48

  • Describe the factors that affect light scatter.
  • Explain how light scatter and fluorescence can be used to detect populations.
  • Describe the use of excitation and emission spectra.
  • Explain what compensation is and why it is necessary for multicolor analysis.

6. Data Analysis – 8:54

  • Describe how data is saved and displayed.
  • Explain the purpose of gating.
  • State examples of data analysis methods.

7. Sorting – 4:25

  • Describe the purpose of sorting cells or particles.
  • Explain how particle size affects the nozzle size and sheath pressure selection.
  • Describe the steps in droplet sorting.

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