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The Polymerase Chain Reaction PDF Free Download

The Polymerase Chain Reaction

The Polymerase Chain Reaction

This book provides an introduction to methods of conducting the polymerase chain reaction (PCR) and the applications of PCR in molecular biology, medicine, and forensics. As a technique, the polymerase chain reaction is revolutionizing the way biomedical research is conducted. It has had a profound impact on areas as diverse as infectious disease diagnosis, identification of genetic mutations responsible for disease susceptibility, and analysis of ancient remains such as those of Neanderthals and mammoths. This book provides a general introduction to PCR in terms of basic principles, equipment, reagents and protocols. The emphasis throughout is on providing enough detail to allow the reader to perform each step successfully. This practical approach continues in the second half of the book where there are chapters on some major applications of PCR including; gene mapping and sequencing; detection of viral infections; identification of bacteria and fungi; analysis of ancient DNA; detection of genetically determined diseases; and forensic applications such as DNA fingerprinting. The final chapter explores some new techniques that have emerged from work on PCR including: the use of inhibitors for DNA RNA isolation from paraffin-embedded tissue sections; application of multiplex PCR for simultaneous

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James D. Watson When, in late March of 1953, Francis Crick and I came to write the first Nature paper describing the double helical structure of the DNA molecule, Francis had wanted to include a lengthy discussion of the genetic implications of a molecule whose struc­ ture we had divined from a minimum of experimental data and on theoretical argu­ ments based on physical principles. But I felt that this might be tempting fate, given that we had not yet seen the detailed evidence from King’s College. Nevertheless, we reached a compromise and decided to include a sentence that pointed to the biological significance of the molecule’s key feature-the complementary pairing of the bases. “It has not escaped our notice,” Francis wrote, “that the specific pairing that we have postulated immediately suggests a possible copying mechanism for the genetic material.” By May, when we were writing the second Nature paper, I was more confident that the proposed structure was at the very least substantially correct, so that this second paper contains a discussion of molecular self-duplication using templates or molds. We pointed out that, as a consequence of base pairing, a DNA molecule has two chains that are complementary to each other. Each chain could then act “. . . as a template for the formation on itself of a new companion chain, so that eventually we shall have two pairs of chains, where we only had one before” and, moreover, ” …

Publisher ‏ : ‎ Birkhäuser; 1994th edition (March 1, 1994)
Language ‏ : ‎ English
Paperback ‏ : ‎ 480 pages
ISBN-10 ‏ : ‎ 0817637508
ISBN-13 ‏ : ‎ 978-0817637507
Item Weight ‏ : ‎ 4.06 pounds
Dimensions ‏ : ‎ 7.01 x 1.09 x 10 inches

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