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The Polymerase Chain Reaction
The Polymerase Chain Reaction
This book provides an introduction to methods of conducting the polymerase chain reaction (PCR) and the applications of PCR in molecular biology, medicine, and forensics. As a technique, the polymerase chain reaction is revolutionizing the way biomedical research is conducted. It has had a profound impact on areas as diverse as infectious disease diagnosis, identification of genetic mutations responsible for disease susceptibility, and analysis of ancient remains such as those of Neanderthals and mammoths. This book provides a general introduction to PCR in terms of basic principles, equipment, reagents and protocols. The emphasis throughout is on providing enough detail to allow the reader to perform each step successfully. This practical approach continues in the second half of the book where there are chapters on some major applications of PCR including; gene mapping and sequencing; detection of viral infections; identification of bacteria and fungi; analysis of ancient DNA; detection of genetically determined diseases; and forensic applications such as DNA fingerprinting. The final chapter explores some new techniques that have emerged from work on PCR including: the use of inhibitors for DNA RNA isolation from paraffin-embedded tissue sections; application of multiplex PCR for simultaneous
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Publisher : Birkhäuser; 1994th edition (March 1, 1994)
Language : English
Paperback : 480 pages
ISBN-10 : 0817637508
ISBN-13 : 978-0817637507
Item Weight : 4.06 pounds
Dimensions : 7.01 x 1.09 x 10 inches
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